![]() ![]() ![]() pEA207 mRNA levels, on the other hand, were resistant to the effects of photobleaching. While transcript levels of the chlorophyll a/b-binding protein decreased by more than 80% under photooxidative light conditions in carotenoid-deficient peas, levels of ferredoxin, the small and large subunits of ribulose 1,5-bisphosphate carboxylase, and pEA214 mRNAs were reduced by less than 50%. Photobleaching was found to affect the expression of the various genes in different ways. The results illustrate a complex set of interactions regulating expression of the nuclear and chloroplast genomes. Relative transcription rates were assayed in isolated nuclei. ![]() mRNA steady state levels of the chlorophyll a/b-binding protein of photosystem II, ferredoxin I, the small and large subunits of ribulose 1,5-bisphosphate carboxylase, and pEA214 and pEA207, two other light-responsive genes, were determined during chlorophyll photooxidation. Changes occurring in several chloroplast components during Norflurazon-induced photobleaching of Pisum sativum seedlings were investigated.
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